Microfluidic lab-on-disc approach of 2,2-diphenyl-1-picrylhydrazyl antioxidant assay / Nurhaslina Abd Rahman

Nurhaslina , Abd Rahman (2019) Microfluidic lab-on-disc approach of 2,2-diphenyl-1-picrylhydrazyl antioxidant assay / Nurhaslina Abd Rahman. Masters thesis, University Malaya.

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Abstract

This dissertation presents the integration of lab-on-disc (LoD) on centrifugal microfluidic platform for the 2,2-diphenyl-1-picrylhydrazyl (DPPH) antioxidant assay. In order to develop the centrifugal microfluidic device, a microfluidic compact disc (CD) was designed and fabricated where the different parallel concentrations, pipetting and loading in the conventional DPPH antioxidant assay procedures was integrated into a single microfluidic CD. The study used two antioxidant standards (ascorbic acid and quercetin) and three local plant extracts (Areca catechu, Polygonum minus and Syzygium polyanthum) to test the developed antioxidant platform. The results showed that, there were a significant difference of p≤ 0.05 between the conventional and LoD method at each concentration among all samples (standard antioxidant and the local plants). The higher the concentration of the extracts, the faster the reaction time for the LoD method to reach the same antioxidant activity level of conventional method. The developed microfluidic CD was able to decrease the conventional method incubation time (30 minutes) with four different parallel concentrations running simultaneously. For ascorbic acid, P. Minus and S. polyanthum it took only 5 minutes (p≤ 0.01) to reach the antioxidant activity level of conventional method, meanwhile for quercetin and A. catechu it takes10 minutes (p≤ 0.01). The developed centrifugal microfluidic CD have offered a rapid and automated platform for DPPH antioxidant assay, it simplified the meticulous and tedious procedures of the conventional DPPH antioxidant assay which can reduced the need of time-consuming and repetitive steps in the manual laboratory operations.

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