DNA-specific schottky diodes for detecting viruses infection in penaeid shrimp / Chan Yen Yew

Chan , Yen Yew (2023) DNA-specific schottky diodes for detecting viruses infection in penaeid shrimp / Chan Yen Yew. PhD thesis, Universiti Malaya.

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Abstract

DNA-based technology has a broad perspective in different applications such as cryptography, information storage, basic logic gates, nanorobots, nanocircuits and nanomedicine as well as in diagnostic. In aquaculture, especially shrimp farming is facing great challenges in preventing bacterial and viral caused diseases which devastates this blooming food industry. However, such problems could be significantly reduced using effective diagnostic methods. DNA diagnostic techniques involves conventional molecular biology diagnostic technique (PCR, LAMP, qPCR, ELISA etc), while new approaches in DNA biosensor (piezoelectric, optical transducer, thermometric and electrochemical) are still being developing. In this work, potential electronic DNA biosensors for electronically characterizing and detecting selected disease-causing viruses is proposed. DNA Schottky diodes are implemented to identify DNA viruses (MBV, IHHNV, WSSV) and RNA viruses (TSV, YHV) in shrimp industry that poses heavy threat to the aquaculture community. With modification of the architecture of the Schottky diode, Al/ITO/DNA/Al and Cu/DNA/Cu are presented to evaluate the effect of DNA and RNA characterization of the viruses. Acquisition and analysis of the current-voltage (I-V) characterization provides the insight of solid-state parameters of the viruses corresponding to the sequence length and polynucleotide arrangement. In a comparison study, selected bacterial DNA employed in the viral characterization study were observed that viruses and bacteria I-V profile showed distinct band structures using Al/ITO/DNA/Al junction. In the solid-state calculation with conventional diode and Cheung’s models, that bacteria profiles possessed higher series resistance and ideality factor while had lower threshold voltage than viral profiles using Al/ITO/DNA/Al junction. These results suggest that the sequence size and polynucleotide arrangement of the DNA sequence affect rectification and charge transport system of DNA biopolymer. Each DNA-specific Schottky diode generated characteristic I-V profiles similar to a semiconducting behavior. DNA-based Schottky diode biosensor showed the rapid biosensing potential to differentiate pathogens from shrimps as the current diagnostic method involves expensive cost and tedious procedures.

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