Improvement of Pleurotus pulmonarius strain through backcrossing breeding method / Wong Yee Ting

Wong , Yee Ting (2024) Improvement of Pleurotus pulmonarius strain through backcrossing breeding method / Wong Yee Ting. Masters thesis, Universiti Malaya.

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Abstract

Pleurotus pulmonarius (Fries) Quélet, commonly known as grey oyster mushroom, is one of the highly commercialised as edible oyster mushroom around the world. The growing market is demanding for new and improved P. pulmonarius hybrids with better yield and quality. The present study was carried out to generate backcrossed (BC1) hybrid of P. pulmonarius through backcrossing of F1 hybrid H4 with recurrent parent PL27 using conventional methods. The backcrossing was aimed to improve the productivity, yield quality and biological efficiency of hybrid with interested morphological traits from the parental strain of P. pulmonarius. A total of 14 fast-growing monokaryons from F1 hybrid H4 were backcrossed with 16 monokaryons isolated from recurrent parent PL27. As result, 67 BC1 hybrid combinations were compatible out of 188 crosses being carried out in this study, with a success rate of 35.6 %. Six BC1 hybrids were selected after evaluation of radial and linear growth study to conduct conventional cultivation method in polybags. Series of morphological measurements and analysis were conducted during cultivation. Four BC1 hybrid were selected for second cycle of cultivation to evaluate the stability of trait performance and heritability of BC1 hybrids. H4(46) X PL27(44) and H4(22) X PL27(43) were chosen as potential BC1 hybrids for successive backcrossing due to consistency in rapid growth potential, large basidiocarp size, high productivity, optimum biological efficiency, low contamination rate and high storage value.

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