Assessment of molecular markers associated with resistance or susceptibility to Fusarium wilt TR4 in Musa acuminata ssp. malaccensis / Fatimah binti Kayat

Kayat, Fatimah (2009) Assessment of molecular markers associated with resistance or susceptibility to Fusarium wilt TR4 in Musa acuminata ssp. malaccensis / Fatimah binti Kayat. PhD thesis, University of Malaya.

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    Abstract

    Bananaandplantain(Musaspp.)arestaplefoodcropsintropicaland subtropicalcountriesandalsoplayakeyroleintheeconomicofmanydeveloping countries.However,thetenyearreview(1996-2005)oftheproductionofthecrop showedthatthelandusageforbananaandplantainhadincreasedbuttheyieldhadbeen reducedduringthesameperiod.InMalaysia,PanamadiseaseorFusariumwiltcaused byFusariumoxysporumf.sp.cubenseVCG01213/16alsoknownasTropicalrace4 (TR4)isthemajorandthemostdestructivediseasewhichcausesconsiderablelossto theMalaysianbananaexporttrade.Conventionalbananabreedingforresistanceto Fusariumwiltishamperedbysterility,triploidy,longgenerationtimeandthelackof appropriatetestingsites.Biotechnologicaladvancesinvolvinginvitropropagation, somaticembryogenesisandmolecularmarkersprovidetoolsforgeneticrelationship studiesandQTLanalysisthuscouldenhancethedevelopmentofcultivarsadaptedto changingenvironments. Theuseofgenesfromthewildcroprelativeshavebeenrecognizedinbreeding programsofmanycrops.Inthisstudy,thestrategyapproachistodevelopnew populationswithtraitsspecifictothepathogenfromtheindigenouswildbananaMusa acuminatassp.malaccensis(AA)whichhasbeenshowntohaveveryhighresistanceto FOCTR4.Maturedseedswereextractedfromfruitbunchesoffourrandomopencross populationsofwildbananainordertoproduceseedprogeniesraisedthroughzygotic embryoculture.Embryogerminationwasachievedwithoutanyseedtreatmentinthe darkfortwoweeksbeforebeingplacedunderlight.Individualseedprogenieswere subjectedtoseveralin-vitrostagesinordertogainuniformplantletswithreplicatesfor diseasescreening.DifferentdegreesofresponsetoFusariumoxysporumf.sp.cubense TR4wereobservedinwildbananaseedprogeniesaswellastheirF1hybridpopulations whichprovidedapotentiallyusefulgeneticresourceforthedevelopmentofdisease resistancemarkersthatcouldbeexploitedinamarker-assistedselection. AhighdegreeofpolymorphisminwildbananaseedprogeniesshownbyRAPD m arkersprovidedgoodearlyevidenceforthepotentialusefulnessofpolymorphismandse gregationstudiesusingmolecularmarkers.AnalysisofRAPD,STMSandAFLPm arkersinthisstudydidnotshowanycleardifferentiationbetweenresistantandsus ceptibleindividuals.However,markersgeneratedbythosemethodswereincludedinl inkagemapdevelopment.Atotalof18markershadbeengeneratedfromRAPDa nalysiswhile14SSRmarkerswereobservedfromSTMSanalysis.Atotalof4657ba ndswasgeneratedfrom30EcoR1+3xMseI+3,oneEcoR1+3xMseI+2and43P stI+2xMseI+3primercombinations.The639markersconsistingof607AFLPs,14S SRsand17RAPDsmarkersweredeterminedontwomappingpopulations(resistancea ndsusceptibilitytoFOCTR4).Atotalof471markers(286Pst-markers,155Eco-m arkers,17RAPDmarkersand13STMSmarkers)wereanalyzedforthesusceptiblem appingpopulationwhile414markers(249Pst-markers,143Eco-markers,9RAPDm arkersand13STMSmarkers)wereanalyzedfortheresistantmappingpopulation.L inkageanalysisofthedataresultedinthegenerationoftwosetsoflinkagemapsc onsistingof32linkagegroupsfortheresistantmappingpopulationand37linkageg roupsforthesusceptiblemappingpopulation.Themapshaveprovidedthebasisoff uturemappingandmarker-assistedbreedingstudiesandstrategiesforthisbanana.

    Item Type: Thesis (PhD)
    Additional Information: Thesis (Ph.D) -- Institut Sains Biologi, Fakulti Sains, Universiti Malaya,2010
    Uncontrolled Keywords: Seed crops--Biotechnology--Research; Bananas--Breeding--Research; Bananas--Diseases and pests--Control--Malaysia; Plantain banana--Diseases and pests--Control--Malaysia; Fusarium oxysporum--Control--Malaysia; Fusarium wilt of banana--Control--Malaysia; Fusarium diseases of plants--Control--Malaysia
    Subjects: Q Science > Q Science (General)
    Q Science > QH Natural history
    Divisions: Faculty of Science
    Depositing User: Mrs Nur Aqilah Paing
    Date Deposited: 27 Sep 2014 11:39
    Last Modified: 27 Sep 2014 11:39
    URI: http://studentsrepo.um.edu.my/id/eprint/4324

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