The application of irap markers in the breeding of papaya / Bathusha Shareef bin Kader Shaik Ali

Kader Shaik Ali, Bathusha Shareef (2013) The application of irap markers in the breeding of papaya / Bathusha Shareef bin Kader Shaik Ali. Masters thesis, University of Malaya.

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    In Malaysia, a papaya breeding program was initiated by the Malaysian Agricultural Research and Development Institute (MARDI) to produce resistant papayas. Nevertheless, selection of resistant papayas from field trials is laborious and time-consuming. In order to facilitate selection of resistant papayas in breeding programs, potential of DNA fingerprinting method was analysed in this study for future applications. Materials from leaves of Carica papaya L. from varieties Tainung 5, Eksotika 6 and their hybrids were used in the assessment of molecular markers. From an analysis of Inter-Retrotransposons Amplified Polymorphism Reaction (IRAP) molecular markers, five combinations out of 45 possible primers combinations showed significant polymorphism in DNA banding patterns. Furthermore, the banding patterns were used to assess the phylogenetic relationship between the papayas sample tested. Out of the five combinations, only one combination of primers showed DNA banding patterns which is similar to the phenotypic segregation of MARDI’s field trial results. IRAP markers could be further tested to be applied in Molecular Assisted Selection (MAS) and Molecular Assisted Breeding (MAB) to facilitate selection of PRSV resistant papayas. For future studies, more IRAP primers can be designed based on Long Terminal Repeat (LTR) sequences of the papaya genome. IRAP markers could also be used in combination with other primer sequences such as from microsatellites. (Retrotransposon-microsatellite Amplified Polymorphism). This is to obtain more informative banding patterns and thus IRAP markers could be fully exploited to facilitate papaya breeding programmes. Hopefully, this would benefit the papaya planting industry.

    Item Type: Thesis (Masters)
    Additional Information: Dissertation (M.Sc.) -- Institut Sains Biologi, Fakulti Sains, Universiti Malaya, 2013
    Uncontrolled Keywords: Irap markers; Breeding of papaya
    Subjects: T Technology > TP Chemical technology
    Divisions: Faculty of Science
    Depositing User: Mrs Nur Aqilah Paing
    Date Deposited: 30 Sep 2014 13:14
    Last Modified: 30 Sep 2014 13:14

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