Phytochemical investigation of Walsura pinnata / Mohamad Azrul Mahdzir

Mohamad Azrul , Mahdzir (2017) Phytochemical investigation of Walsura pinnata / Mohamad Azrul Mahdzir. Masters thesis, University of Malaya.

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Abstract

Ten compounds were successfully isolated from the dichloromethane extract of bark of Walsura pinnata Hassk which was collected at 243 km, from Gua Musang, Kelantan to Kuala Lipis, Pahang. A new oleanane triterpenoids; 3-oxo-olean-9(11),12-dien-28-oic acid (100) was established along with nine known compounds, which are five terpenoids, 3-oxo-lup-20(29)-en-28-oic acid (97), 3β-hydroxy-5-glutinen-28-oic acid (98), 3-oxo-olean-11-en-28,13β-olide (99), 3-oxo-20(24)-epoxy-12β,25-dihydroxydammarane (101), 2(3),6(7)-diepoxy-9-humulene (102), two sterols compounds, stigmasterol (103), β-sitosterol glycoside (104) with two aromatic derivatives, which are 4-hydroxy-3-methoxybenzoic acid (105) and 4-hydroxy-4,8-dimethyl-1-tetralone (106) were analyzed and confirmed using various spectroscopic techniques. Compound 97 and 98 were tested in vitro for their cytotoxic activities against five type of cancer cell lines; human hepatocellular carcinoma (Hep3b and HepG2), human ovarian adenocarcinoma (SK-OV-3), human breast adenocarcinoma (MCF-7), and human acute myeloblastic leukemia (Kasumi-1). Compound 97 showed good cytotoxicity against leukemia, Kasumi-1 cell line with IC50 value of 2.11 ± 0.48 μg/mL while compound 98 exhibit good cytotoxicity activities against both liver cancer cell lines, Hep3b (IC50: 2.28 ± 0.00 μg/mL , SI = 8.81) and HepG2 (IC50: 4.00 ± 0.63 μg/mL, SI = 5.02).

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