Abdolmalaki, Rojin (2013) Acute toxicity and anti-ulcer mechanisms of Teucrium zanonii extract against Ethanol-induced gastric mucosal injuries in rats / Rojin Abdolmalaki. Masters thesis, University Malaya.
Abstract
The study was performed to assess the anti-ulcer activity of Teucrium zanonii leaves ethanolic extract against ethanol induced gastric ulcer in rat model. Apart from that, acute toxicity, antibacterial and antioxidant activities of T. zanonii ethanolic extract were evaluated. Experimental groups were orally administered with different concentrations of T. zanonii ethanolic extract (50, 100, and 200 mg/kg) diluted with 10% Tween 20 solution. Negative control group (ulcer control) was pretreated with vehicle only (10% Tween 20). Positive control (reference group) was orally administered with 20 mg/kg Omeprazole. One hour after administration, animals in all groups received absolute ethanol to generate gastric mucosal injuries. An hour later, all rats were sacrificed and the ulcerous areas of the gastric wall were ascertained. The ulcer control group showed intense mucosal injuries while pretreatment with T. zanonii indicated high protection level against gastric mucosal injuries, enhancing the production of mucus and flattening of gastric mucosa. Histological evaluation of gastric wall in ulcer control group revealed acute damage of gastric mucosa along with edema and leucocytes infiltration in submucosal layer compared to pretreated groups with T. zanonii. Acute toxicity test up to 5 g/kg of this extract did not display any toxicological symptoms in rats. The antibacterial study of T. zanonii did not demonstrate any antibacterial activity against B. subtilis, E. coli, Staph. aureus, P. aeruginosa, Klebsiella pneumonia and Methicillin-resistant Staphylococcus Aureus (MRSA). In addition, T. zanonii showed low antioxidant activity compare to quercetin, ascorbic acid, gallic acid and butylated hydroxytoluene. In brief, the findings of this study proposed that protective effect of T. zanonii against gastric ulcer was obviously tangible with comparative diminished ulcerative area, declining of edema and leucocytes infiltration in submucosal layer.
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