Ansari, Mahsa (2013) Isolation and characterization of indigenous microorganisms from agricultural soil / Mahsa Ansari. Masters thesis, University of Malaya.
Abstract
Microorganisms have many properties include the ability to degrade waste products. In this study Haemolysis was used as an initial selection criterion for the primary isolation of surfactant-producing bacteria. Only β and γ Haemolytic activity were detected in this study which as the presence of a definite clear zone around a colony. From this study, Out of 139 (49 %) hemolytic colonies were observed in this study had shown strong haemolytic activity. The Gram stain results had showed: cocci and bacilli shapes. The colony morphology identification had shown rough, smooth and mucoid appearance. 120 of 139 (86%) isolates possessed biocleaner activity with emulsification against crude oil. Susceptibility towards antibiotics of 138 soil bacterial isolates were tested with impregnated discs (Oxoid) by the Kirby-Bauer method. Inhibition zones were assessed on Mueller-Hinton agar and evaluated after 18 and 24 hours of incubation at 37°C. The disc antibiotic result had shown 29 different types of antibiogram patterns. The lysis solution test could be used as a tool for differentiation between gram positive and gram negative bacteria. All isolates are plasmidless and mice virulence test (LD50) had shown no virulence occurred in all colonies tested.
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