Cheng, Poh Guat (2014) Wound healing effects of aqueous extracts of Ganoderma Lucidum (m. a. Curtis: Fr.) Pers karst in Streptozotocin-induced diabetic rats / Cheng Poh Guat. Masters thesis, University of Malaya.
Abstract
There are about 180 million diabetic patients in the world and this figure is increasing at an alarming rate. One of the complications due to diabetes is impaired wound healing. About 15% of Type-2 diabetic patients suffer from diabetic foot ulcer (DFU) and 84% of them have their lower leg amputated. Type-1 represents 5 to 10 per cent of diabetes mellitus patients. To date to our knowledge, there is limited success in DFU treatment. The objective of this study was to investigate the effects of Ganoderma lucidum aqueous extract in accelerating wound healing in normal and diabetic rats. The freeze-dried powder of aqueous extract of G. lucidum was investigated for the rate of healing and wound closure in normal and diabetic rats. Six groups of streptozotozin-induced (Type-1 diabetes) Sprague Dawley rats were wounded with 2cm2 diameter wound in the dorsal neck region. The wound of negative control animals were treated with aqueous cream, while Intrasite gel was applied to the wounds of positive control animals. The wound of rats in treatment groups were dressed with aqueous cream containing 5%, 10%, 15% and 20% (w/w) aqueous extract of G. lucidum. Aqueous cream containing the aqueous extract of G. lucidum significantly, (p< 0.05) accelerated the rate of wound healing compared to wounds dressed with aqueous cream alone. Aqueous cream containing 10% (w/w) aqueous extract showed the best wound closure quality among the treatment groups. The healed tissue was excised and histological staining was performed using H&E (Haematoxylin & Eosin) and MT (Masson Trichrome) staining to further confirm the healing. Wounds dressed with aqueous cream containing 10% G. lucidum extract showed markedly less scar width at the wound closure, had large numbers of fibroblast proliferation, more mature and densely packed collagen with accompanying angiogenesis in the healed tissue compared to wounds dressed iii with aqueous cream alone. These results were indicative of the beneficial effects of G. lucidum on wound healing and closure in diabetic rats. Furthermore, aqueous extract of G. lucidum increased antioxidant capacity in both serum of normal and diabetic rats through external application and the advanced oxidation protein products (AOPP) and lipid hydroperoxides products (LHP) in diabetic rats were due to the untreated diabetes during the period of experiment.
Actions (For repository staff only : Login required)
![[img]](http://studentsrepo.um.edu.my/style/images/fileicons/application_pdf.png)
